PEmax-enRERV(for mammalian cell)

LOCUS       PEmax-enRERV(for mammalian cell)        9825 bp DNA     circular SYN 27-JUN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    PEmax-enRERV (for mammalian cell)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 27, 2026 from SnapGene 7.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        270..649
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        650..853
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        895..913
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             963..983
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             984..5084
                     /codon_start=1
                     /label=nCas9
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5142..5162
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5187..7247
                     /codon_start=1
                     /label=enRERV
                     /note="Insert Sequence"
                     /translation="PPVACLVLSLEEEYRLHEQNPKQLLAPEWLSAFSEVWAEQTGMGL
                     AKQVPPVVVELKADASPVSVKQRPMSREAKEGIRPHIQRLLQLGILVPCQSPWNTPLLP
                     VRKPGTNDYRPVQDLREVNKRVQDIHPTVPNPYNLLSSLPPERTFYTVLDLKDAFFCLR
                     LHPNSQPLFAFEWRDPEGGHTGQLTWTRLPQGFKNSPTLFNEALHRDLAPFRAQNPQIS
                     LLQYADDLLLAASTQELCREGTKRLLNELGELGYRVSAKKAQLCRTEVTYLGYTLREGK
                     RWLTEARKKTVMQIPTPTTPRQVREFLGKAGFCRLFIPGFATLAAPLYLLTKPKVPFTW
                     TEEHQRAFEDIKAALLAAPALALPDLTKPFTLYVDERAGVARGVLTQALGPWKRPVAYL
                     SKKLDPVASGWPSRLKAIAAVALLVKDADKLTLGQHVTVIAPHALESIVRQPPDRWMTN
                     ARMTHYQSLLLNERVTFAPPAILNPATLLPEIHNSTPIHQCVDILAEETGTRKDLTDRP
                     WPGVPAWYTDGSSFVVEGKRRAGAAVVDGKQVIWASSLPEGTSAQKAELVALTQALRLA
                     EGKAINIYTDSRYAFATAHIHGAIYKQRGLLTSAGRDIKNKEEILALLEAVHLPKKVAI
                     IHCPGHQKGEDPITKGNQMADLVAKQVAQQVTILAEKSQTPVKTPVTDDSNY"
     CDS             7293..7313
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7323..7349
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             7358..7375
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    7404..7628
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7699..7715)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7723..7739
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7747..7777)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7792..7813
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8101..8689)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8860..9720)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9721..9825)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcgat ctcccgatcc
       61 cctagggtcg actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
      121 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
      181 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
      241 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
      301 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
      361 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      421 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      481 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      541 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      601 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      661 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      721 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      781 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      841 tataagcaga gctggtttag tgaaccgtca gatccgctag agatccgcgg ccgctaatac
      901 gactcactat agggagagcc gccaccatga aacggacagc cgacggaagc gagttcgagt
      961 caccaaagaa gaagcggaaa gtcgacaaga agtacagcat cggcctggac atcggcacca
     1021 actctgtggg ctgggccgtg atcaccgacg agtacaaggt gcccagcaag aaattcaagg
     1081 tgctgggcaa caccgaccgg cacagcatca agaagaacct gatcggagcc ctgctgttcg
     1141 acagcggcga aacagccgag gccacccggc tgaagagaac cgccagaaga agatacacca
     1201 gacggaagaa ccggatctgc tatctgcaag agatcttcag caacgagatg gccaaggtgg
     1261 acgacagctt cttccacaga ctggaagagt ccttcctggt ggaagaggat aagaagcacg
     1321 agcggcaccc catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccca
     1381 ccatctacca cctgagaaag aaactggtgg acagcaccga caaggccgac ctgcggctga
     1441 tctatctggc cctggcccac atgatcaagt tccggggcca cttcctgatc gagggcgacc
     1501 tgaaccccga caacagcgac gtggacaagc tgttcatcca gctggtgcag acctacaacc
     1561 agctgttcga ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg
     1621 ccagactgag caagagcaga aagctggaaa atctgatcgc ccagctgccc ggcgagaaga
     1681 agaatggcct gttcggaaac ctgattgccc tgagcctggg cctgaccccc aacttcaaga
     1741 gcaacttcga cctggccgag gatgccaaac tgcagctgag caaggacacc tacgacgacg
     1801 acctggacaa cctgctggcc cagatcggcg accagtacgc cgacctgttt ctggccgcca
     1861 agaacctgtc cgacgccatc ctgctgagcg acatcctgag agtgaacacc gagatcacca
     1921 aggcccccct gagcgcctct atgatcaaga gatacgacga gcaccaccag gacctgaccc
     1981 tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta caaagagatt ttcttcgacc
     2041 agagcaagaa cggctacgcc ggctacattg acggcggagc cagccaggaa gagttctaca
     2101 agttcatcaa gcccatcctg gaaaagatgg acggcaccga ggaactgctc gtgaagctga
     2161 agagagagga cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc ccccaccaga
     2221 tccacctggg agagctgcac gccattctgc ggcggcagga agatttttac ccattcctga
     2281 aggacaaccg ggaaaagatc gagaagatcc tgaccttccg catcccctac tacgtgggcc
     2341 ctctggccag gggaaacagc agattcgcct ggatgaccag aaagagcgag gaaaccatca
     2401 ccccctggaa cttcgaggaa gtggtggaca agggcgcttc cgcccagagc ttcatcgagc
     2461 ggatgaccaa cttcgataag aacctgccca acgagaaggt gctgcccaag cacagcctgc
     2521 tgtacgagta cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg accgagggaa
     2581 tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc catcgtggac ctgctgttca
     2641 agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt
     2701 gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc ctgggcacat
     2761 accacgatct gctgaaaatt atcaaggaca aggacttcct ggacaatgag gaaaacgagg
     2821 acattctgga agatatcgtg ctgaccctga cactgtttga ggacagagag atgatcgagg
     2881 aacggctgaa aacctatgcc cacctgttcg acgacaaagt gatgaagcag ctgaagcggc
     2941 ggagatacac cggctggggc aggctgagcc ggaagctgat caacggcatc cgggacaagc
     3001 agtccggcaa gacaatcctg gatttcctga agtccgacgg cttcgccaac agaaacttca
     3061 tgcagctgat ccacgacgac agcctgacct ttaaagagga catccagaaa gcccaggtgt
     3121 ccggccaggg cgatagcctg cacgagcaca ttgccaatct ggccggcagc cccgccatta
     3181 agaagggcat cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc
     3241 acaagcccga gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc cagaagggac
     3301 agaagaacag ccgcgagaga atgaagcgga tcgaagaggg catcaaagag ctgggcagcc
     3361 agatcctgaa agaacacccc gtggaaaaca cccagctgca gaacgagaag ctgtacctgt
     3421 actacctgca gaatgggcgg gatatgtacg tggaccagga actggacatc aaccggctgt
     3481 ccgactacga tgtggacgct atcgtgcctc agagctttct gaaggacgac tccatcgaca
     3541 acaaggtgct gaccagaagc gacaagaacc ggggcaagag cgacaacgtg ccctccgaag
     3601 aggtcgtgaa gaagatgaag aactactggc ggcagctgct gaacgccaag ctgattaccc
     3661 agagaaagtt cgacaatctg accaaggccg agagaggcgg cctgagcgaa ctggataagg
     3721 ccggcttcat caagagacag ctggtggaaa cccggcagat cacaaagcac gtggcacaga
     3781 tcctggactc ccggatgaac actaagtacg acgagaatga caagctgatc cgggaagtga
     3841 aagtgatcac cctgaagtcc aagctggtgt ccgatttccg gaaggatttc cagttttaca
     3901 aagtgcgcga gatcaacaac taccaccacg cccacgacgc ctacctgaac gccgtcgtgg
     3961 gaaccgccct gatcaaaaag taccctaagc tggaaagcga gttcgtgtac ggcgactaca
     4021 aggtgtacga cgtgcggaag atgatcgcca agagcgagca ggaaatcggc aaggctaccg
     4081 ccaagtactt cttctacagc aacatcatga actttttcaa gaccgagatt accctggcca
     4141 acggcgagat ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt
     4201 gggataaggg ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc caagtgaata
     4261 tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa agagtctatc ctgcccaaga
     4321 ggaacagcga taagctgatc gccagaaaga aggactggga ccctaagaag tacggcggct
     4381 tcgacagccc caccgtggcc tattctgtgc tggtggtggc caaagtggaa aagggcaagt
     4441 ccaagaaact gaagagtgtg aaagagctgc tggggatcac catcatggaa agaagcagct
     4501 tcgagaagaa tcccatcgac tttctggaag ccaagggcta caaagaagtg aaaaaggacc
     4561 tgatcatcaa gctgcctaag tactccctgt tcgagctgga aaacggccgg aagagaatgc
     4621 tggcctctgc cggcgaactg cagaagggaa acgaactggc cctgccctcc aaatatgtga
     4681 acttcctgta cctggccagc cactatgaga agctgaaggg ctcccccgag gataatgagc
     4741 agaaacagct gtttgtggaa cagcacaagc actacctgga cgagatcatc gagcagatca
     4801 gcgagttctc caagagagtg atcctggccg acgctaatct ggacaaagtg ctgtccgcct
     4861 acaacaagca ccgggataag cccatcagag agcaggccga gaatatcatc cacctgttta
     4921 ccctgaccaa tctgggagcc cctgccgcct tcaagtactt tgacaccacc atcgaccgga
     4981 agaggtacac cagcaccaaa gaggtgctgg acgccaccct gatccaccag agcatcaccg
     5041 gcctgtacga gacacggatc gacctgtctc agctgggagg tgactccggc ggaagctctg
     5101 gtggcagcaa gcggaccgcc gacggctctg aattcgagag ccctaagaag aaaagaaagg
     5161 tgagcggagg ctctagcggc ggaagccctc ctgtggcctg cctggtctta agcctcgagg
     5221 aggaataccg cctgcatgag cagaacccca agcagctcct ggccccagaa tggttgagcg
     5281 ctttctctga ggtctgggct gagcaaactg gaatgggcct ggccaagcag gtgccccctg
     5341 tcgtagtgga gctgaaggct gacgctagcc cagtgtcagt gaagcagaga cccatgtcta
     5401 gagaagccaa ggagggcatc cggccacaca tccagcggct tcttcagttg ggcatcctgg
     5461 taccctgcca gtctccctgg aacactccgc tgctgcctgt gaggaagcct gggaccaatg
     5521 actataggcc tgtccaggat ttacgggaag tgaacaaaag agtacaagac attcacccta
     5581 ctgtgcccaa cccctacaat ttgctgagct ctctgcctcc tgagcgcaca ttctacacag
     5641 tcctggacct caaggatgcc ttcttctgcc tccgcctgca tccaaactcc cagccactct
     5701 tcgcctttga gtggagagac ccagagggag gccacacagg ccagctgacg tggactcggc
     5761 tgccccaggg tttcaaaaac tccccgactc tgtttaatga agctctgcac agggacctgg
     5821 cgcctttccg ggctcaaaac ccacaaatct cattactgca gtacgctgat gatttgctgc
     5881 tagctgcgtc cactcaggag ttgtgtagag aaggcaccaa gagactgctg aatgaactcg
     5941 gagagctcgg ctacagggtg tccgcgaaga aagctcagct gtgcaggact gaggtgacct
     6001 acctcggcta cactctccgg gagggtaaac gttggctgac agaggcccga aagaagacag
     6061 tcatgcagat ccctacccct acgacccccc gtcaggtgcg ggagttcctg gggaaggcag
     6121 gcttctgtag actcttcatc ccaggctttg ctaccctggc agcacctctg tacctgctga
     6181 ctaagccgaa ggtgccattc acttggacag aagaacacca gagagcgttt gaagacataa
     6241 aagccgccct cctggcagcc ccagccctag ccctcccaga cctgaccaag ccattcaccc
     6301 tgtatgtgga tgagagagct ggcgttgccc gtggtgtact tacccaggcc cttggcccgt
     6361 ggaagagacc cgtggcatac ttaagtaaga agctggaccc tgtcgccagt ggctggccaa
     6421 gcagactcaa ggccattgca gctgtggctc tgctcgtgaa ggatgctgac aagcttacgc
     6481 tgggccagca tgtgaccgtg atcgctcccc acgccctaga gtctattgtc cggcagccac
     6541 ccgataggtg gatgaccaac gcaaggatga ctcactatca gtcactgtta ctgaatgaga
     6601 gggtgacgtt tgctcctcct gcgatcttga atcctgcaac cctgctcccc gaaatccaca
     6661 atagcacacc catccatcag tgtgtggaca tcttggccga agaaacaggc actagaaagg
     6721 accttacaga ccggccttgg cctggagtgc ctgcctggta cactgatggc agttcctttg
     6781 tggtagaggg caaaagaaga gccggcgcag ctgtagttga tggcaaacag gtcatctggg
     6841 ccagcagcct accagaaggg acgtctgcac agaaagcaga gctggtggcc ctgactcaag
     6901 ccctgcgtct ggcggaagga aaggccatca acatttatac agactcccgc tatgccttcg
     6961 ccacagccca cattcatggg gccatctaca aacagcgagg cttactgacg tcagctggaa
     7021 gagatatcaa aaacaaggag gagatcctgg cccttctgga agctgtccac cttcctaaaa
     7081 aggttgccat tattcactgc cctgggcacc agaagggaga agatcccata accaagggaa
     7141 accagatggc agacctagtg gctaaacagg tggcccagca ggtgaccatc ctggcggaaa
     7201 agtctcaaac ccccgtgaaa acacctgtta cagatgacag caactattct ggcggctcaa
     7261 aaagaaccgc cgacggcagc gaattcgagt ctcccaagaa gaagaggaaa gtcggctctg
     7321 gccctgccgc taagagagtg aagctggact aaccggtcat catcaccatc accattgagt
     7381 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     7441 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     7501 tgagaaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     7561 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     7621 ctctatggct tctgaggcgg aaagaaccag ctggggctcg ataccgtcga cctctagcta
     7681 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7741 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct aatgagtgag
     7801 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7861 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7921 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7981 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     8041 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8101 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8161 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8221 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8281 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8341 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8401 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8461 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8521 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     8581 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8641 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8701 gatcttttct acggggtctg acactcagtg gaacgaaaac tcacgttaag ggattttggt
     8761 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8821 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8881 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8941 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     9001 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     9061 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     9121 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     9181 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     9241 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     9301 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     9361 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     9421 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     9481 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     9541 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9601 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9661 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9721 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9781 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcg
//